Characterization And Chemistry Of Imidazolidinyl Urea And Diazolidinyl Urea PdfBy Plexerouthar1954 In and pdf 25.04.2021 at 03:18 3 min read
File Name: characterization and chemistry of imidazolidinyl urea and diazolidinyl urea .zip
- Imidazolidinyl urea
- Effects of Several Cosmetic Preservatives on ROS-Dependent Apoptosis of Rat Neural Progenitor Cells
- Formaldehyde and Formaldehyde-Releasers
- Diazolidinyl urea
Formaldehyde has many applications, which are shown in tabular format in this publication. However, it is a ubiquitous and important allergen. Contact allergy to formaldehyde occurs frequently in women with hand dermatitis.
Die vorliegende Erfindung beschreibt auch ein Verfahren zur Stabilisierung der antigenen Stellen von Zellen oder Zellkomponenten. Die vorliegende Erfindung beschreibt weiterhin Verfahren zur Nutzung von Zellen oder Zellkomponenten in histologischen Untersuchungen und dergleichen. The The present invention generally relates to solutions, the composition which are used to stabilize and fix cells and tissues, and in particular a composition for stabilization and fixation of cells and tissues for a suitable period of time conserve antigenic sites.
The present invention describes also a method for stabilizing the antigenic sites of Cells or cell components. The present invention describes Furthermore, methods of using cells or cell components in histological examinations and the like.
Entsprechend werden diese biologischen Materialien oft in histologischen, cytologischen, immunologischen und Protein-Untersuchungen und dergleichen verwendet. This biological Materials are often used in a wide variety of applications, including but not limited on teaching aids and the diagnosis and treatment of diseases, used.
For example, certain organisms and components thereof often in or through solutions conserved for use in the teaching areas. Become accordingly these biological materials often in histological, cytological, immunological and protein studies and the like. Consequently is it desirable in the art to obtain a fixing or stabilizing solution, which not only let use so that all Results are accomplished by a previous fixing or stabilizing solution available are but also to provide a solution can preserve the antigenic sites.
The Tasks of any "preservative", that in the fixation or stabilization of biological materials is used, change and hang from the intended use of the "conserved" material, however, it is in the state the technique of certain materials to secure some desirable Results.
The invention thus relates to compositions for fixation of cells and tissues and methods of fixing cells and Tissues using certain reagents as fixatives. The primary The task of tissue fixation is to provide as much as possible many structural details of cells and their components. To this Purpose the cells in their original one unchanged Morphology are kept so that maximum cell detail can be observed to let.
There is also this in the clinical application of immunostaining Requirement that the antigens are not affected by the fixation or Stabilization process changed become. The microscope is still the conventional one Means for examining fixed and dyed biological materials, however, you can biological materials also with a flow cytometer to be examined.
The flow cytometer is an important procedure for Examination of a large number of cells in a short time. The usual Formulations for stabilizing cells include an or several agents that react violently with the proteins of the cells, so that the cell components are denatured and insolubilized. Typical for this Kind of agent are picric acid, Mercury ions, formaldehyde and glutaraldehyde.
In addition, can be Some less toxic compounds use the proteins denature and stabilize, such as acetic and formic acid. Unfortunately the use of these compounds is less because of their toxicity as satisfactory. Therefore, it is highly desirable to use fixatives develop safely, efficiently and appropriately in histological examinations can be used.
In der Technik sind ebenfalls verschiedene Verfahren zur Analyse von histologischen, cytologischen, immunologischen und proteinhaltigen Materialien bekannt. Various techniques are also known in the art for the analysis of histological, cytological, immunological and proteinaceous materials known.
EP A relates to mounting compositions for use in delaying the fading of preparations stained with a fluorochrome for immunofluorescence, which composition may comprise 1,4-diazobicyclo [2,2,2] octane in a suitable solvent.
EP A1 relates to the preservation of biological samples with a mixture containing hydantoin, Dowil 75, methylparate, neocebitat and aluminum sulphate using an alcohol and ethylene glycol to dissolve the paraffin. GB A relates to a fixative for histological, cytological, immunological and proteinaceous preparations containing a mixture of pyrolidone, a polyol, a urea and a zinc salt of an nm-oxidizing organic or inorganic acid.
The US Patent relates to a preservative composition, comprising a mixture of a formaldehyde donor and a halopropynyl compound. The Surface marker analysis has developed into a laboratory tool that is particularly suited to the clinical Diagnosis by examination of immunodeficient states, differentiation of cell types and developmental stages and other cell processes.
The expansion of uses for surface marker analysis it lead to, flow cytometry and antibody probes for evaluating cell characteristics be used. Although there are other means of surface marker analysis, however, flow cytometry represents a rapid, objective and quantitative determination of surface markers ready. Durchflusszytometer arbeiten im Prinzip durch Multiparameter-Analyse von heterogenen Zellpopulationen oder Zellkomponenten auf der Grundlage von Zelle zu Zelle. The flow cytometry allows biological and determined physical properties of cells and cell components can be.
Bei der Durchflusszytometrie werden Zellen in Suspension in einer Einzelreihe durch hydrodynamisches Fokussieren durch den Weg eines Laserstrahls geleitet. Eine Reihe von Linsen sammelt das gestreute oder ausgestrahlte Licht und leitet es zu einem Photomultiplier. Jeder Photomultiplier misst einen gesonderten Parameter. The interaction of the cells with the laser beam scatters a part of the light and causes an excitation and the emission of fluorescent molecules, the on the surface the cell or in its interior are present.
A series of lenses Collects the scattered or emitted light and directs it a photomultiplier. Each photomultiplier measures a separate one Parameter. The measured parameters include: forward light scattering, which measures the relative particle size; Side light scattering, which is the relative granularity or another internal Structure measures; and the fluorescence emission.
The optical signals be from a computer into a data display for analysis and Interpretation transformed. One Chromophore can be added to the cell suspension, or the cells can with monoclonal antibodies which are conjugated directly or indirectly with fluorochromes are.
These probes are ordinary for a surface antigen or some intracellular Substance of interest, such as DNA, specific. The choice of the probe depends on the diagnostic or biological parameter of clinical interest from. Compounds commonly used to stabilize cells may conserve some cellular antigens, although certain antigens are more sensitive to the loss of reactivity to monoclonal antibodies. Examples of such antigens are the cluster designation antigens CD present on the surface of hematopoietic cells.
Much of the clinically appropriate applications of surface marker analysis, and much of the development of surface marker testing technology The focus is on lymphocyte CD markers. One Another problem with the use of common compounds, the cells To stabilize analysis, that is certain critical antigens are present in small quantities, so they are undetectable even if only a low percentage of these antigens destroyed becomes.
Therefore It is an object of the invention to provide a fixative solution for tissue and cells that are extremely low toxicity has and yet all Requirements of a model fixing agent met.
A Another object of the invention is to provide a fixative solution for tissue and cells, the tissues and cells and their cellular details preserved. A Another object of the invention is the provision of a fixing agent, the additional to a low toxicity no harmful fumes is emissive, non-flammable or carcinogenic and that safe and sound can be disposed of properly.
Yet Another object of the invention is to provide a fixer for tissue and cells that conserve tissues and cells and their antigenic details, thus immunohistochemical and other immunological in the cells and tissues Techniques can be performed satisfactorily.
Yet Another object of the invention is to provide a fixer the one unchanged antigenic surface for reaction with specific antibodies for use, for example in the manufacture of vaccines and related immunotherapeutic Method supplies. Noch eine weitere Aufgabe der Erfindung ist die Bereitstellung von cytologischen Kontrollen zur Verwendung bei biologischen Analysen und Untersuchungen.
Toxicity studies, the diazolidinyl urea according to the invention with formaldehyde of the prior art, show the following:.
These reduced toxicity reduces the problem of disposal and handling. As it also no toxicity when inhaled, no badge detection devices are required, as for Formaldehyde.
One another advantage offered by the active compounds according to the invention, is the fact that they are not flammable and therefore not flammable, as are many of the prior art fixatives. Man nimmt an, dass der Wirkstoff auf eine bestimmte Weise an die Zellmembran oder das Gewebe bindet. The Mechanisms by which the active compounds of the invention provide the desired stabilization of the tissue and the cell membrane are not exactly accurate known.
It is believed that the active ingredient in a certain way binds to the cell membrane or tissue. This hypothesis will because many of the active compounds according to the invention are known disinfectants are to kill the bacteria by bind them to cell structures.
This is not a complete explanation for the mechanism the for the results of the invention is responsible, as many others Disinfectants, such as Kathon and Omadin, no tissue and cell stabilizing Cause effects. Formaldehyd vernetzt mit sich selbst und mit Proteinen und verbirgt das Antigen. Zur Bestimmung, ob sich dieses bewahrheitet, wurde Diazolidinyl-Harnstoff zu dem Protein Albumin hinzugegeben. The ability the active compounds according to the invention for the preservation of antigens is also not understood however, it may be based on a difference in the reaction between proteins and the active compounds according to the invention, compared with prior art fixatives such as formaldehyde.
To determine whether this is true, diazolidinyl urea became added to the protein albumin. After incubation of the mixture from diazolidinyl urea and protein for 24 h. If this experiment is done with formaldehyde, it comes to a big one Number of multimers and insoluble Proteins. It It has been found that the addition of glycine or a other formaldehyde-reactive compound for the elimination of any free formaldehyde which is an equilibrium component the compositions of the invention can be.
It should be noted, however, that the preferred Compositions only trace amounts of formaldehyde in equilibrium contain. Die vorliegende Erfindung kann ebenfalls mit einer beliebigen Zellkomponente oder einem biologischen Material, das mindestens eine antigene Stelle aufweist, verwendet werden.
The present invention can also be used with a any cell component or a biological material that has at least one antigenic site, can be used. In another aspect of the invention, it has been discovered that the fixative of the present invention can be used in the preparation of vaccines and related immunotherapeutic procedures.
Thus, a vaccine comprising an antigen treated with the fixative of the present invention delt, in a biocompatible form suitable for administration in vivo, and methods of administering the same to a recipient in an amount sufficient to enhance the immune response, also provided in accordance with the invention. It has also been discovered that in vivo administration of the fixative of the present invention produces an effective immunogen, and thus also provides a method of "boosting" an in vivo immune response in a recipient by administering the fixative of the invention in a biocompatible form which is suitable for administration in vivo in an amount sufficient to enhance the immune response is provided in accordance with the invention.
The term "biocompatible form suitable for administration in vivo" means a form to be administered, the therapeutic effects of immunotherapy outweighing the toxic effects, if any. In addition, the administration may be in a suitable pharmacological form which includes but is not limited to intravenous injection. The active ingredients according to the invention are known formaldehyde donors. It is not understood yet however, the small amount of formaldehyde is in equilibrium with the reagent is not known to be the active mechanism the compositions of the invention.
Es zeigen: The Various advantages of the present invention will become apparent to those skilled in the art upon reading the following description and appended claims and with reference to the following drawings. Show it:. The include fixer solutions according to the invention the active ingredients in a solvent, selected from water, dimethyl sulfoxide, alcohol and mixtures thereof.
The Alcohol solvents comprises one or more alkanols, such as methanol, ethanol, propanol, Butanol, polyols, for example diols or triols, such as ethylene glycol, Glycerol, propylene glycol and trimethylene glycol and mixtures of Alkanols and polyols. It is also preferred that a suitable buffer, such as citrate buffer, to adjust the pH to about too the solution is added.
A particularly preferred citrate buffer for use in the solution is sodium citrate dihydrate, but other buffers can be used use, as the expert knows. Whether the solvent used is water, alcohol, dimethylsulfoxide or a mixture thereof depends in principle on the fixed tissue or the fixed membrane. For example, when fixing large pieces of tissue, it is preferable to use an alcohol solvent or an aqueous alcohol solvent because alcohol solvents increase penetration.
When fixing cells, such as pap smears, alcoholic preparations are preferred because they cause the cells to adhere to the slides. If aqueous alcoholic solutions are used as solvents for the active compounds according to the invention, this is due to Ratio of alcohol to water in the range of 4: 1 to 2: 1.
The Amount of the active ingredients in the formulation according to the invention is the one for fixing or stabilizing the tissue or the cell membrane is effective. Usually include the compositions in an exemplary embodiment about 20 to 40 g of 2-bromonitropropane-1,3-diol Bronopol more preferably about 30 g and about 10 to 15 g of water-soluble zinc salt especially preferably about 12 g per ml of solvent used.
Especially preferred become zinc sulfate, and even stronger preferably zinc sulfate heptahydrate is used as the water-soluble zinc salt, however, a number of other zinc salts are also suitable, as one of ordinary skill in the art will appreciate White.
For example, you can also zinc salts, such as zinc chloride or zinc acetate, are used, however, these are considered less effective than zinc sulfate.
In addition to the zinc salt, preferably about 2 to 6 g of citrate buffer more preferably about 3 g , such as sodium citrate dihydrate for the above fixer given. In an exemplary embodiment is about 0. The dissolved Substances in the preparations according to the invention can also contain another additive conventionally to histological fixation preparations is given. These additives include mordants, buffers, penetration enhancers, osmotics active substances and enhancers for the details of the nucleus and core size enhancers.
Das bevorzugte Salz ist Zinksulfat.
Effects of Several Cosmetic Preservatives on ROS-Dependent Apoptosis of Rat Neural Progenitor Cells
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For several decades, the cosmetic preservatives imidazolidinyl urea (IU) and diazolidinyl urea (DU) have not only been poorly characterized.
Formaldehyde and Formaldehyde-Releasers
Diazolidinyl urea is the essential component in the condensation product of allantoin with formaldehyde and a so-called formaldehyde releaser. The compound is used as a biocide to preserve cosmetics and personal care products. The target product differs in the substitution pattern on the imidazolidine ring from the previously stated structure of a 1- [1,3-bis hydroxymethyl -2,5-dioxoimidazolidinyl] -1,3-bis hydroxymethyl urea. The now obsolete structural formula can still be found in many publications.
Die vorliegende Erfindung beschreibt auch ein Verfahren zur Stabilisierung der antigenen Stellen von Zellen oder Zellkomponenten. Die vorliegende Erfindung beschreibt weiterhin Verfahren zur Nutzung von Zellen oder Zellkomponenten in histologischen Untersuchungen und dergleichen. The The present invention generally relates to solutions, the composition which are used to stabilize and fix cells and tissues, and in particular a composition for stabilization and fixation of cells and tissues for a suitable period of time conserve antigenic sites. The present invention describes also a method for stabilizing the antigenic sites of Cells or cell components.
Use: Diazolidinyl urea is widely used as antiseptic for its bactericidal effect.
Imidazolidinyl urea is an antimicrobial preservative used in cosmetics [ citation needed ]. It is chemically related to diazolidinyl urea which is used in the same way. Imidazolidinyl urea acts as a formaldehyde releaser. Some people have a contact allergy to imidazolidinyl urea causing dermatitis. Imidazolidinyl urea was poorly characterized until recently and the single Chemical Abstracts Service structure assigned to it is probably not the major one in the commercial material.
Embed Size px x x x x For several decades, the cosmetic preservatives imidazolidinyl urea IU and diazolidinyl urea DU have not only been poorly characterized but have also had misleading chemical structures assignedto them. This publication gives an insight into what these 2 well-known contact allergens consistof and their degradation patterns. A full chemical characterization of compound HUis shown.
Боль в боку немного утихла, да и глаза как будто обрели прежнюю зоркость. Он немного постоял, наслаждаясь ярким солнцем и тонким ароматом цветущих апельсиновых деревьев, а потом медленно зашагал к выходу на площадь. В этот момент рядом резко притормозил мини-автобус. Из него выпрыгнули двое мужчин, оба молодые, в военной форме.
Свет в бывшем гимнастическом зале выключили. Пьер Клушар спал глубоким сном и не видел склонившегося над ним человека. Игла похищенного у медсестры шприца блеснула в темноте и погрузилась в вену чуть выше запястья Клушара. Шприц был наполнен тридцатью кубиками моющего средства, взятого с тележки уборщицы.